AMINO ACIDS & PROTEINS

 

Amino Acids

Enzymes (like other proteins) are polymers made from the 20 amino acids. An amino acid is a molecule with an amino group and a carboxylic acid group. The amino group of one reacts with the carboxylic acid of another as they become linked forming a protein. Each of the 20 has its own "personality" determined by its sidechain. A few examples:

glycine the smallest allows the most freedom of rotation

electrostatic surface
lipophilic surface

asparagine polar

electrostatic surface
lipophilic surface

phenylalanine nonpolar aromatic large

electrostatic surface
lipophilic surface

aspartate negatively charged

electrostatic surface
lipophilic surface

lysine positively charged

electrostatic surface
lipophilic surface

Complete list

PROTEINS

There are two major ways that the protein polymer will fold in on itself, as a helix or as a sheet.

You may find this tutorial using Protein G which has both structures useful. Much of the protein shape is determined by hydrophobic structures burying themselves in the middle of the protein.

You shouldn't think of the proteins as static structures but rather as waving and jiggling (sort of like the dancing baby)

Enzymes

Enzymes are proteins which catalyze specific reactions by binding certain molecules (substrates) and then sterically and electronically distorting them. The active site of an enzyme is where the substrates are bound and distorted.

 

 

 

hydrophobic pocket
gly-ala-tyr substrate
backbone
ser195
his 57
asp 102
oxyanion hole
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