From Microbes to Mice: Solving Problems with Magnetic Resonance
The Wellesley College Science Center Our Summer Team What is Nuclear Magnetic Resonance (NMR)? What is Magnetic Resonance Imaging (MRI)? Outline of NMR Spectroscopy Projects and MRI Projects NMR Spectroscopy Project: N metabolism of Cyanobacteria Cyanobacteria Response to Environmental Stress Factors Major Endogenous Nitrogen Stores of Cyanobacteria Goals of Our Cyanobacteria Projects 1H–15N Spin-Coupling to Compare Incorporation of 14N and 15N What is Phycocyanin? Molecular Structure of Phycocyanin What is Phycocyanobilin? Proton NMR Spectrum of Phycocyanobilin What is Cyanophycin? 1H NMR Spectrum of Cyanophycin What happens when Cyanophycin is formed under stressful conditions? 1H NMR Spectra of CGP from cells grown on 14NO3– treated with CM in presence of 15NO3– Rate of 15N vs. 14N Incorporation Pieces of the Puzzle Hypotheses Based on Data and Literature MRI Projects Tracing Neural Tracts Using MnCl2 in Crustacean Brain Sequential MRI "Slices" through Normal Mouse Brain Research Support		Major endogenous nitrogen stores of cyanobacteria: Biliproteins (phycobiliproteins), such as phycocyanin (blue pigment) Found in phycobilisomes of cyanobacteria and red algae Blue accessory pigment attached to photosynthetic membranes Accounts for up to 20% of proteins in cyanobacteria Cyanophycin a/k/a multi-L-arginyl-poly(L-aspartic acid), arg-poly(asp), or CGP Found in cyanophycin granules Unique (almost) to cyanobacteria
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Susan D. Kunk Nancy H. Kolodny August 28, 2003 September 11, 2003