The Wellesley College Science Center

From Microbes to Mice: Solving Problems with Magnetic Resonance

The Wellesley College Science Center
Our Summer Team
What is Nuclear Magnetic Resonance (NMR)?
What is Magnetic Resonance Imaging (MRI)?
Outline of NMR Spectroscopy Projects and MRI Projects
NMR Spectroscopy Project: N metabolism of Cyanobacteria
Cyanobacteria Response to Environmental Stress Factors
Major Endogenous Nitrogen Stores of Cyanobacteria
Goals of Our Cyanobacteria Projects
¹H–15N Spin-Coupling to Compare Incorporation of ¹⁴N and ¹⁵N
What is Phycocyanin?
Molecular Structure of Phycocyanin
What is Phycocyanobilin?
Proton NMR Spectrum of Phycocyanobilin
What is Cyanophycin?
¹H NMR Spectrum of Cyanophycin
What happens when Cyanophycin is formed under stressful conditions?
¹H NMR Spectra of CGP from cells grown on ¹⁴NO₃^– treated with CM in presence of ¹⁵NO₃^–
Rate of 15N vs. 14N Incorporation
Pieces of the Puzzle
Hypotheses Based on Data and Literature
MRI Projects
Tracing Neural Tracts Using MnCl₂ in Crustacean Brain
Sequential MRI "Slices" through Normal Mouse Brain
Research Support

The Wellesley College Science Center

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Susan D. Kunk
Nancy H. Kolodny
August 28, 2003
September 11, 2003