How to Tune and Match the Easy Way
  1. Log on in the normal fashion. If the green iconnmr windows come up, exit to unix by choosing that option under the File menu on the top of the iconnmr window. At this stage, you should see a blue unix window. If you just have icons for unix windows (usually labeled winterm), you might have to double click to open one.
  2. Within a blue unix window, type "xwinnmr" at the % prompt to start the nmr program.
  3. Put in your sample. This is done by typing "ej" on the pink command line to eject the old sample, then "ij" to inject your sample.
  4. Make a datafile and load parameters to tell the spectrometer which nuclei you will be studying. This can be done by typing "edc" and filling in the data file information, or by recalling a dummy file with the command "re junk 1". That only works if junk 1 exists, so the first time you do this you might have to use edc to make junk 1. But afterwards, use the "re junk 1" method…it saves disk space!
  5. Load the parameters by typing "rpar" on the pink line. Choose appropriate parameters from the list that pops up. They are alphabetical, first by capitals (Bruker standards) and then lower case (ours). Obviously you should choose a carbon set (like C13CPD) to tune for carbon & protons and a silicon set (like si_inept) to tune for silicon & protons. After you choose a parameter set, you will get another window with options for subsets of parameters (acquisition, processing, etc.) Just click on Copy All.
  6. Next type "wobb" to start the tune process. Once it says it has started (look below the command line for a report) type "acqu" to see the visual display. You must use the control sliders on the base of the probe to center the dip and get it all the way down. Get the appropriate starting values for the slider positions from the card attached to the probe. Once you have the dip well-positioned and you are satisfied with it, press the "Channel Select" button ON THE BOX BY THE MAGNET to look at the proton tune & match. In a minute the proton display will come up on the computer screen, and you can use the rods to tune & match for protons (again, center & lower the dip). The rods are stiff, and there is a tool to turn them with. DO NOT FORCE THEM!
  7. When you are done, type "stop" on the pink line. Then type "iconnmr" and proceed as normal with your experiment.
  8. At the end, you must set the tune & match back to carbon. So choose "exit to xwinnmr" from the File menu on the iconnmr interface, put the sealed sample in the magnet, load carbon parameters (C13CPD), and tune & match again. Basically you are just repeating steps 3-6 on this list. When you are done, exit the xwinnmr program by choosing the Exit option from the File menu. You might get a warning that processes are still active. If so, say yes, it is okay to exit anyway. Finally, log out of the system by putting the cursor somewhere in the background area, holding down the RIGHT mouse button, and choosing the Logout option.