WELLESLEY COLLEGE

Recombinant DNA and Biological Agent Project Renewal Form

Please update your recombinant DNA and biological agents project for review and reapproval by the Institutional Biosafety Committee.  Write “no changes” if no change.  Attach additional sheet if neded.  Return to Harry Clark.  Thank you.

Principal Investigator:__________________________________________________________________________________
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Project Title:_________________________________________________________________________________________

Current Associate Investigators (list):

For recombinant experiments, list new host cells (if E.coli, is it a K12 strain?   If cell line what species source, is it primary or established?), new vectors (with host range), new DNA inserts (species source, if viral - % of genome inserted.  Is protein expression sought?  If expressed, is the protein expressed physiologically active?  Or a toxin?  If toxin, what is the LD 50?)

List nonrecombinant biological agents in use (e.g., human blood, tissue, cell lines, other microorganisms):

List lab room numbers used and posted containment levels:

               
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PI  Signature                                                                                  Date