Figure 2

Antisense to SRC-1 & CBP mRNA Decreases
Estrogen-dependent PR Gene Expression in the VMN

Control Antisense
Using an antisense technique to decrease the expression of the nuclear receptor coactivators, SRC-1 and CBP, in the brains of female rats reduces estrogen receptor transcriptional activity in the ventromedial hypothalamus, a brain region involved in hormone dependent female reproduction.  Treatment with antisense to SRC-1 and CBP reduces the number of progestin receptor cells (brown labeled cells) that are induced by activated estradiol-bound estrogen receptors. (Molenda et al., 2002)